diff --git a/support/config-fragments/autobuild/bootlin-nios2-glibc.config b/support/config-fragments/autobuild/bootlin-nios2-glibc.config
deleted file mode 100644
index 575a1b30ed..0000000000
--- a/support/config-fragments/autobuild/bootlin-nios2-glibc.config
+++ /dev/null
@@ -1,4 +0,0 @@
-BR2_nios2=y
-BR2_TOOLCHAIN_EXTERNAL=y
-BR2_TOOLCHAIN_EXTERNAL_BOOTLIN=y
-BR2_TOOLCHAIN_EXTERNAL_BOOTLIN_NIOS2_GLIBC_STABLE=y
diff --git a/support/config-fragments/autobuild/toolchain-configs.csv b/support/config-fragments/autobuild/toolchain-configs.csv
index 654f11e28b..047b22a9fa 100644
--- a/support/config-fragments/autobuild/toolchain-configs.csv
+++ b/support/config-fragments/autobuild/toolchain-configs.csv
@@ -28,7 +28,6 @@ support/config-fragments/autobuild/bootlin-m68k-5208-uclibc.config,x86_64
 support/config-fragments/autobuild/bootlin-microblazeel-uclibc.config,x86_64
 support/config-fragments/autobuild/bootlin-mipsel-uclibc.config,x86_64
 support/config-fragments/autobuild/bootlin-mipsel32r6-glibc.config,x86_64
-support/config-fragments/autobuild/bootlin-nios2-glibc.config,x86_64
 support/config-fragments/autobuild/bootlin-openrisc-uclibc.config,x86_64
 support/config-fragments/autobuild/bootlin-powerpc64le-power8-glibc.config,x86_64
 support/config-fragments/autobuild/bootlin-powerpc-e500mc-uclibc.config,x86_64